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A blinded researcher gave paw scorings (score ~ 0-4) based on the following criteria: 0, normal

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Methylglyoxal-mediated anxiolysis involves increased protein modification and elevated expression of glyoxalase 1 in the brain

Of course, NO molecules group can also be transferred directly to the protein to undergo modification, and the dynamic balance of S-nitrosothiols also relies on the thioredoxin/thioredoxin reductase (Trx/TrxR) system, where Trx-mediated reduction of SNO proteins to thiols is at the expense of oxidation of NAPDH, which ultimately and generates NO to mediate the relevant signaling pathway