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Samples were incubated at 80 C for overnight
Although degradation-synthesis coupling would be consistent with Figures 3A,B, it is also unexplained why metabolism of GABA should be so important for glutamate production, since GABA in adult animals contributes so relatively little to the return flux toward astrocytes ( V cyc )
The experiment was conducted in quadruplicate, and each replicate contained two pots
