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Alexa Fluor conjugated secondary antibodies were used to fluorescently label the antigens of interest and following dehydration in a series of increasing concentrations of methanol and cleared using dibenzyl ether, the membrane-bound slices were placed onto a glass microscope slide and imaged using a Zeiss LSM-800 inverted confocal microscope

This metabolic shift occurs due to the peptides action on the nicotinamide methylation pathway , which increases intracellular NAD+ availability, facilitating greater oxidative metabolism

Several studies have demonstrated a link between VEGFR-2 and the ERK pathway, where VEGFR-2 acts upstream of ERK phosphorylation (186188)