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The standard reconstitution workflow for most research peptides using bacteriostatic water is straightforward: allow the lyophilised peptide vial to reach room temperature, add the appropriate volume of bacteriostatic water using a sterile needle and syringe, swirl gently (do not shake vigorously, as this can cause peptide aggregation or degradation), and allow to stand until fully dissolved

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An increased intestinal PEG-400 permeability in first-degree healthy relatives of Crohn's disease patients was described for the first time in 1986 (107), whereas three other studies could not reproduce these findings by using the same probe (108110)

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