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First, side-chain carboxyl groups and hydroxyl groups must be protected as amides or esters

Cytokine change may induce alterations in neurotransmitter systems and neuronal integrity by inducing excitotoxic glutamate receptor-mediated damage, influencing monoaminergic systems [serotonin (5-HT), DA, and NE], -aminobutyric acid (GABA), acetylcholine, neuropeptides, and BDNF, which are directly associated with cognitive function and neurodegenerative processes (Wang et al., 2015)

Stained membranes were washed three times with TBS-T and stained with HRP-linked secondary antibody donkey-anti-rabbit (Amersham, NAV934) 1:5,000 in BSA 2.5% for 1 h

For each sample, two biological replicates were analyzed with three technical replicates