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The hemolysis ratio was quantified by measuring supernatant absorbance at 545 nm using a microplate reader
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[1] [2] [3] [20] NAD+ supplementation could prevent mitochondrial deterioration and preserve metabolic function/energy (ATP) generation by balancing age-related degradation of NAD+ and its precursor nicotinamide mononucleotide via NADases, particularly CD38
