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Real-time polymerase chain reaction (PCR) was performed with Takyon Rox SYBR master mix (Eurogentec, Seraing, Belgium) in a StepOnePlus (Applied Biosystems, Life Technologies, Foster City, CA, USA) using the following primers: IL-6 F 5ACTGGCAGAAAATAAGCTGAATCTTC 3 and R 5TGATCAAGCAAATCGCCTGAT3

New Research Discovers Evidence of Atypical & Classical ME/CFS - The Microbe Discovery Project - Apr 4, 2017 Brenu, Ekua Weba

All subjects provided informed consent for long-term storage and sharing of biospecimens and data per the original observational cohort study protocol

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