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Description
The hereromeric GluK2/K5em receptor was expressed in HEK293S GnTI cells using the BacMam system, solubilized in 100 mM dodecyl--D-maltoside (DDM, Anatrace), 0.5% cholesterol hemisuccinate (CHS, Anatrace), followed by sequential Twin-Strep (GluK2) and 1D4 (GluK5) affinity purification and Superose 6 size-exclusion chromatography 23

Additionally, it is most effective when combined with proper nutrition and lifestyle changes
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