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28 , S12 (2004)

10.3171/jns.2002.97.6.1302 J Neurosurg 43 KapralovA

Rosen MJ, Karns R, Vallance JE, Bezold R, Waddell A, Collins MH, et al

For immunoprecipitation, cells were harvested and lysed in NP-40 lysis buffer (50 mM HEPES, 150 mM NaCl, 10% glycerol, 1% NP-40, 1 mM EDTA, 1 mM EGTA, pH 7.4) supplemented with protease and phosphatase inhibitor cocktail