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coli cells were resuspended in buffer containing 20 mM Tris-HCl (pH 7.5), 0.50 M NaCl, 0.50 M MgCl 2 , 10 mM 2-mercaptoethanol and 1 mM phenylmethanesulfonyl fluoride (PMSF), and were disrupted with an ultrasonic homogenizer

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After that, cells were washed with phosphate-buffered saline (PBS) and incubated with XTT reagent kit as recommended by the manufacturers instructions (Roche, Basel, Switzerland)

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