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Financial Disclosure: The authors declared that this study has received no financial support

Wide-field prism-type total internal reflection fluorescence (TIRF) microscopy was used with a solid-state 532nm diode laser to generate an evanescent field of illumination to excite the fluorophores (Cy3 or Cy5) at the sample chamber

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It is usually located behind the outer mitochondrial membrane and therefore separated from the nucleus
