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D-Penicillamine versus zinc sulfate as first-line therapy for Wilsons disease

The percentage of proteins related to lipid catabolic process, organic acid catabolic process, cellular amino acid metabolic process, fatty acid metabolic process, and Golgi vesicle transport was significantly higher in the Down group while the percentage of proteins involved in biopolymer biosynthetic process, gene expression, RNA processing, biopolymer modification, phosphorus metabolic process, cell-cell signaling, and cell proliferation was significantly higher in the Up group

The cell pellet was resuspended in Buffer A (25 mM Tris-HCl, pH 8), 500 mM NaCl, 10% glycerol, 20 mM imidazole) and lysed by sonication on ice