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Protein concentrations were quantified using a BCA protein quantification kit according to the manufacturers protocol (Thermo Fisher Scientific, Rockford, U.S.A.), and equal amounts of proteins (~30 g) were resolved on 10%12.5% SDS-PAGE gels

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Research demonstrates BPC-157 works primarily through activation of the VEGFR2 (vascular endothelial growth factor receptor 2) and modulation of nitric oxide signaling via the Akt-eNOS pathway