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Binding of Protein on GST Beads Incubate the soluble cell lysate with 1 ml of GST beads (prewashed two times with 10 ml of GST binding buffer) for 1 hr at 4 C under gentle rotation
Furthermore, targeted secondary prevention or immunomodulatory interventions should be explored based on immune phenotypes, thereby achieving precision management through a risk stratification-immune-guided intervention-dynamic immune monitoring-chronic injury prevention approach

Cell Death Differ 16 , 13031314 (2009)

& Jung, K