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The conditions inside the phagosome of activated macrophages will shift towards glycolysis, which convert glucose mostly to primary substrates of Krebs cycle in anaerobic conditions of activated macrophage, macrophages use up large amount of glucose in short time after activation and then the source of energy will change to glycerol ( IcL inside activated macrophage suggests that the loss of capacity to survive and clearance of IcL mutants might start as early as macrophages get activated at the site of infection ( Analysis of cytokine gene expression by RT-PCR in the calf model indicated that the expressions of proinflammatory cytokines were mostly upregulated and higher than the wild-type group in the BacA group at 12 weeks post-inoculation

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Importantly, the current evidence gap should not be interpreted as evidence of ineffectiveness