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Western blot analysis was performed using standard procedures, which was detected using an enhanced chemiluminescence western blotting detection kit (32106, Thermo Fisher), and was quantified by scanning densitometry according to the manufacturers protocols

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2 Materials and methods 2.1 Animal experiment and sampling The study was conducted at the experimental station of the Institute of Animal Nutrition, Friedrich-Loeffler-Institut (FLI), located in Braunschweig, Germany, in full compliance with the German Animal Welfare Act and approved by the Lower Saxony State Office for Consumer Protection and Food Safety (LAVES, Oldenburg, Germany) (AZ33.19-42502-04-16/2378)

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