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Protein Purification by HisTrip Column and 6XHis Tag TEV Cleavage Frozen bacterial pellets were resuspended in the lysis buffer (50 mM Tris-HCl, 150 mM NaCl, pH 8.0), and the cells were lysed using an ultrasonic processor
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In addition, administration of GH by the continuous infusion of hormone for 6 days in hypophysectomized rat liver has no effect on mitochondrial respiration and enzyme activities (Mutvei et al., 1989)
