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These transgenic mice showed significantly reduced plasma testosterone levels (18% of normal)[124] and the IGF-I deficiency was correlated with an ultrastructural analysis of mutant Leydig cells revealing a significant developmental delay, with fewer and smaller Leydig cells than normal

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Metabolites produced by the gut microbiota during growth play crucial roles in maintaining intestinal barrier integrity, facilitating host nutrient absorption, balancing the intestinal microenvironment, and regulating metabolism and immunity (337)

The substrates for these reactions are both endogenous (intrinsic) and exogenous (xenobiotics), being mainly electrophiles, Michael acceptors, epoxides, electron-withdrawing structures and carbonyls, which are able to react through nucleophilic additions with GSH