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Rapid and high-sensitive phosphoproteomics elucidated the spatial dynamics of the mouse brain

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The gradient elution was set as follows: 10% B at 0 min and held for 0.2 min, increased to 50% B at 0.4 min and held for 3.8 min, then decreased to 10% B at 4.5 min followed by 5 min for equilibration
