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Once a portion of the original H 2 O 2 substrate has been consumed by GPx, any remaining H 2 O 2 reacts with Fe 2+ ions from the FAS via a Fenton-type redox mechanism, oxidizing Fe 2+ to Fe +
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Conversely, the increased activity of Nrf2 is effective for the prevention of chemical carcinogenesis
A representative example of gating strategy used to characterize FASN lox/lox -PyMT MEFs in apoptosis in 2D cultures, by using co-staining with Annexin-V and DAPI is shown in Supplementary Fig
