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Description
Finally, the fermentation was scaled up in a 5-L fermentor, and the highest titer and yield were improved to 5680 mg/L and 45.13 mg/g, respectively, by optimizing the addition conditions of amino acids (40 mM added after 40 h)
The no-NAC group sustained perfusions for an average of 96 (range 87102) h, universally developing methaemoglobinaemia (2%) observed after an average of 45 h, with subsequent steep rise
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Ingredients: Thiaminehydrochloride 100 mg; monothioglycerol 0.5%; Water forInjectionq.s. Sodium hydroxide may have been added for pH adjustment (2.5 to 4.5)
