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Description
Convenient, cost-effective, optimal ratio
The cell lysate was mixed with the purified recombinant protein and Ni-NTA or GSH beads and incubated at 4 C for 25 min, and the bound fraction was eluted with 400 mM imidazole or 100 mM GSH in Elution Buffer (100 mM NaCl, 50 mM HEPES, 0.2 mM DTT

Effects of rimonabant, a cannabinoid CB1 receptor ligand, on energy expenditure in lean rats

10.1093/nutrit/nuz092 Nutr
