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2011;6(2) doi: 10.1371/journal.pone.0014661.e14661 [DOI] [PMC free article] [PubMed] [Google Scholar] 61.Bailey HH, Ripple G, Tutsch KD, et al

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First, we utilized the CRISPR-Cas9 system to generate Acsl4 knockout MTECs and confirmed the knockout efficiency via Sanger sequencing and Western blot analysis (Fig