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It is also possible that generation of nicotinamide by CD38 may regulate SIRTUINs activity, since nicotinamide is an endogenous inhibitor of the SIRTUINs [62-63]

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Although a 2,7-dichlorodihydrofluorescein diacetate probe has been previously used to measure ROS levels in 661 W cells 43 to further confirm the increase in ROS formation under light exposure, an alternative method using the CellROX Green reagent has been assayed