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While all those data indicate that MOR phosphorylation would play a crucial role in desensitization, Qiu and collaborators showed that a truncated mutant of MOR from S363 is able to undergo a similar desensitization to the wild type demonstrating that receptor phosphorylation is not an absolute prerequisite for desensitization (Qiu et al., DOR

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Cells were left to adhere for 90 min at room temperature followed by an overnight incubation at 37 C, 5% CO 2