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Microleakage assessment Assessment of linear dye penetration at tooth- restoration interface both occlusal and gingival was done using the stereomicroscope at 100X magnification (MA 100 Nikon stereomicroscope Japan with Omnimet image analysis software) to study the degree of microleakage in micrometers
Common regimens include 50 mg/kg intraperitoneally twice daily for 1228 days in C57BL/6J or ob/ob mice, with indirect calorimetry and body-composition endpoints

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