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Description
It is synthesized from three amino acids in two ATP-dependent steps, beginning with the formation of a peptide bond between -glutamate and cysteine by -glutamylcysteine synthetase (GSH1) and the subsequent addition of glycine, catalyzed by glutathione synthetase (GSH2) (Figure 1: GSH biosynthesis) [20]
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Tracheotomy was performed and the animals were allowed to breathe spontaneously through the tracheal cannula
