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In another study, ectonucleoside triphosphate diphosphohydrolase-1 (ENTPD1) was upregulated in AraC resistant AML which was associated with enhanced mitochondrial activity ( 3.1 Therapy-induced adaptations in TCA cycle proteins and metabolites Although Otto Warburg found that cancer cells may rely on glycolysis rather than oxphos for their energetic needs, studies in more recent years have found that certain cancers may preferentially utilize oxphos rather than glycolysis

Similar results were obtained in cultured cells, where added acetylcarnitine (2 mM) contributed about 10% of the acetyl-CoA used for de novo synthesis and for elongation of fatty acids, as well as 6% for ketogenesis [117]

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Self-amplifying mRNA (saRNA) is an alternative approach to mRNA vaccines, and has been shown to induce immune responses with lower doses of saRNA than mRNA (10- to 100-fold lower) [15], and results in prolonged protein expression for up to 60 days in vivo [4]