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Autotomy behavior correlates with the DRG and spinal expression of sodium channels in inbred mouse strains

Membranes were probed overnight at 4 C with primary antibodies against p-c-Met (3077 T, Cell signaling), c-Met (8198 S, Cell signaling), p-AKT (AF0016, affinity), AKT (GR100134-1, abcam), p-ERK (AF1015, affinity), ERK (4695 T, Cell signaling), GAPDH (10494-1-AP, proteintech), p-TRKB (4603 T, Cell signaling), CyclinD1 (26939-1-AP, proteintech), cleaved caspase-3 (9661 L, Cell signaling), caspase-3 (66470-2-Ig, proteintech), N-cadherin (22018-1-ap, proteintech), E-cadherin (AF0138, Beyotime), -actin (A5441, Sigma), AXL (4977, CST), p-MER (AF8443, affinity), P-AXL (334580, abmart), NF-KB (10745-1-AP, proteintech), p-ALK (3341 S, CST), Ki67 (27309-1-AP, proteintech)

It was not brief, routine, or limited to preparing for the procedure

This guide cuts through that with the clinical protocols Beyond Stem Cells actually uses, the math behind the doses, and the safety boundaries that matter