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Escherichia coli sequence type ST131 as the major cause of serious multidrug-resistant E

Akagi D, Chen M, Toy R, Chatterjee A, Conte MS

doi:10.3390/nu13061913

2.10 Transmission electron microscopy (TEM) Fresh kidney tissue was cut into small pieces and incubated in fixing solution at 4 C for 4 h in preparation for transmission electron microscopy, followed by secondary fixation at room temperature using 0.1 mol/L of PBS containing 1% osmium tetroxide for 2 h