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Glutamine is an essential fuel for rapidly dividing cells, such as enterocytes, fibroblasts, and leukocytes because it is a precursor of peptides, proteins, nicotinamide adenine dinucleotide phosphate (NADPH), antioxidants, purines, and pyrimidines ( When glutamine concentration lowers under one of the stressful conditions mentioned above, cells, such as lymphocytes, macrophages, and neutrophils, have their function and performance impaired due to the lack of their primary source of fuel

After washes with PBS for 3 times, cells were incubated with 1 M DAF-FM DA probe for 20 ~ 30 min

Food Chemistry, 245, 168177

(2022) 28:gaac018