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Hoechst 33342 staining of the bovine blastocyst (Figure 6A) was also applied to assess the developmental potentials of cryopreserved bull semen with the cell number/blastocyst of each group analyzed
Proliferation was measured using the Cell Titer GLO assay (Promega), according to the manufacturers instructions
Table 4 Characteristics of the studies in this review
, Rahman K., Kamal A
