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(2011) proposed a sandwich layer method in which colonies from soil samples are first cultured on medium A, then the whole agar of medium A is lifted from the plate and transferred to the surface of medium B (containing the methylene blue indicator), thus forming a medium A-colonies- medium B sandwich. After incubation for 2 h at 40C, strains forming haloes are then picked and purified

10.1200/JCO.2005.03.0106 [DOI] [PubMed] [Google Scholar] Van Cutsem E., Prenen H., D'Haens G., Bennouna J., Carrato A., Ducreux M., et al

However, DBS is also associated with potential side effects