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Notable CRP increases (above 50100 mg/L) are associated with advanced stages/metastasis and poor prognosis, but the importance of hsCRP is still unknown with no proven value
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Heat-induced epitope retrieval (HIER) pretreatments were applied using BOND Epitope Retrieval (ER) Solutions citrate-based pH 6.0 ER1 or EDTA-based pH 9.0 ER2 (both Leica Biosystems)
