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Among these are the (i) production of osmolytes, (ii) removal of the toxic salt ion Na + and Cl or their compartmentalization into vacuoles, and (iii) upregulation of antioxidant mechanisms to eliminate excessive reactive oxygen species (ROS) [5, 7,8,9]

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Association between subclinical hypothyroidism and depression: an updated systematic review and meta-analysis

To remove some precipitated or denatured substances, the following method is recommended: Wash with 2 column bed volumes of 6M guanidine hydrochloride solution, followed immediately by 5 column bed volumes of PBS, pH 7.4