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Fixed oocytes were blocked in 7% goat serum (v/v) and 1% BSA (w/v) prepared in PBS/0.1% Tween-20 (PBST) for 1 h at room temperature

Pentose Phosphate Pathway + ratio excess NADH Inhibits citric acid cycle Citric acid cycle The citric acid cycle, also known as the tricarboxylic acid (TCA) cycle or the Krebs cycle, is a cyclic set of reactions that occurs in the mitochondrial matrix

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Figure 9 3.9 Determination of spiked recoveries The results of the spiked recovery experiments were shown in Table 2, the recoveries of this method were from 76.94 to 99.48%, which were in accordance with the AOAC regulations