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doi: 10.4236/fns.2014.511112 31
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When the finely-tuned trafficking of macromolecules is dysregulated in genetically susceptible individuals, both intestinal and extraintestinal autoimmune disorders can occur.[105] There is growing evidence that increased intestinal permeability plays a pathogenic role in various autoimmune diseases
Site-directed mutagenesis and in vitro enzyme assays and quantification of FNSII proteins Each site-directed mutagenesis version of the target proteins was prepared as described according to the manual for the Fast Mutagenesis System (Transgen, China) and subcloned into the pYeDP60 vector
