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In lung cancer, ROS generated by cigarette smoke cause DNA mutations and contribute to the activation of pro-inflammatory cytokines that promote tumorigenesis

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Fifty microliters of samples or 8-oxoG standards were added to the wells of the 8-oxoG conjugate-coated plate and incubated for 10 min at room temperature on an orbital shaker

Research has shown that in mice with high oxalate urine and oxalate-stimulated HK-2 cells, there is a significant increase in ROS levels (206), indicating that ferroptosis may be involved in the formation of kidney stones