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Autolytic changes during frozen storage The reduction of trimethylamine oxide (TMAO), an osmoregulatory compound in many marine teleost fish, is usually due to bacterial action (section 5.3) but in some species an enzyme is present in the muscle tissue which is able to break down TMAO into dimethylamine (DMA) and formaldehyde (FA): (CH 3 ) 3 NO (CH 3 ) 2 NH + HCHO It is important to note that the amount of formaldehyde produced is equivalent to the dimethylamine formed but is of far greater commercial significance