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Description
The cells were next subjected to media containing L-Azido-homoalanine (AHA, 50 M, 4 h), to allow AHA incorporation into nascent proteome (see Methods)

Parabens & Phthalates Parabens (like methylparaben or propylparaben) are chemical preservatives used to extend the shield life, while Phthalates are used to make synthetic fragrances stick to your skin longer
Now you need to draw accurate doses from the vial

All animal experiments were conducted in accordance with the NIH Guide for the Care and Use of Laboratory Animals or an equivalent standard that meets or exceeds the ethical and welfare requirements outlined in the NIH Guide
