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Study approval All protocols were approved by the Institutional Animal Care and Use Committee at University of California Davis and are in accordance with the National Institutes of Health guidelines for care and use of laboratory animals Results Arrestin-3 engagement improves morphine-induced analgesia and reduces morphine tolerance We measured morphine-induced analgesia with a radiant heat tail-flick assay in three different mouse genotypes: WT, in which MOR poorly engages arrestin-3 in response to morphine [14], Arr-3 KO [16] and knock-in mice with a mutant MOR that robustly recruits arrestin-3 (RMOR) [13]

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SSRIs aim to restore the natural balance of serotonin, a neurotransmitter that affects mood