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Absorbance at 450 nm was measured using a microplate reader (M5

Interestingly, the researchers stratified the heart failure group by diabetes status and found C2 to be increased in the diabetic subgroup

Thus, for dogs and cats with cancer, supplementation may be necessary when there is no optimal supply of this vitamin in the diet, with an intake of 4.4 g per 1,000 Kcal of metabolizable energy being recommended for adult cats and 9.6 g per 1,000 Kcal of metabolizable energy for adult dogs (175)