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278 Additionally, a casein-derived peptide enhanced the TER value, indicating an increase in GI barrier function, and increased the levels of occludin gene and protein expression in in vitro human intestinal epithelial Caco-2 cells, as well as in ex vivo mouse jejunal and ileal loops

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Increased TNF levels, associated with NFKB, have also been observed in WD patients [29] and animal models [14]

The choice of timing should therefore prioritise consistency and individual tolerance rather than attempting to optimise absorption through scheduling