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After incubation with the secondary antibody, the cells were washed with PBST and mounted using a Fluoroshield Mounting Medium with DAPI (Abcam ab104139)

We have a little bit of clarification , like the longer term, higher dose are going to cause more risk of developing neurodevelopmental disorders down the road in our children

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ROS acts as upstream signals for NLRP3 inflammasome activation by upregulating the expression of key components, including NLRP3, pro-CASP1, and pro-IL1B [141]