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Blood was collected in red-top tubes, centrifuged at 500 g for 10 min and serum was aliquoted into cryopreservation tubes and stored at 80 C until shipped to Metabolon for metabolomics analysis

This not only enhances nutrient and oxygen delivery to working muscles but also contributes to the sought-after muscle pump effect during workouts

Cholinergic hypothesis The cholinergic hypothesis was the earliest to delineate the pathogenesis of AD

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