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Do not increase your dose prematurely or abandon the protocol because you do not see immediate results
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Acknowledgments This work was partially supported by NIH 5UG3DA047717 (H.M.M
Waltham, MA, USA) staining as adapted and modified from published protocols.[12] Briefly, cells were washed twice with PBS, fixed with 10% formalin for 30 min at room temperature, and stained with oil red O working solution (~0.2% oil red O in 99% isopropanol) for 30 min
