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For instance, vitamin B2 (riboflavin) and the essential trace mineral selenium are necessary for the recycling [the enzyme glutathione reductase is considered a flavoenzyme (i.e., it needs a coenzyme form of riboflavin, flavin adenine dinucleotide to operate)] of and use [the enzyme glutathione peroxidase is a selenoprotein (i.e., it needs its cofactor selenium to operate)] of glutathione, respectively

To measure UGA recoding as a selenocysteine, we established a GFP-Reporter Of Selenocysteine Insertion (gROSI), in which a cysteine71 in the GFP was replaced with a UGA coupled with the 3UTR GPX4 Selenocysteine Insertion Sequence (SECIS) required for UGA readthrough as a selenocysteine 34 (Figure 1H)

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