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To optimize results, its recommended to stay hydrated and avoid intense physical activity for 24 hours
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In brief, EIA microplates (Corning CLS3590) were coated overnight with 100 l of coating buffer (carbonate-bicarbonate with pH = 9.6, Sigma C3041) or phage Muddy diluted to 5 10 9 pfu ml 1 in coating buffer
