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Further, treatment with increased concentration of insulin, a potent physiological activator of the PI3K/Akt pathway, rescued the normal level of expression of PPAR, the master regulator of adipogenesis, and overcame the restraining effect of menadione on the differentiation capacity of 3T3-L1 preadipocytes

F s is the observed fluorescence of the sample well, F b is the fluorescence of the blank well and F c is the fluorescence observed in the control well

Appropriate dilutions of DMSO, R001 or H 2 O 2 standard in KRPG buffer were added to the cells at 50 L per well for 048 h

Potential risks include infusion side effects, long infusion times, and high costs