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Chatzakis, C

Discussion In general, CxxC/S-type Grxs function primarily in redox regulation and electron supply to metabolic enzymes, CGFS-type Grxs in FeS cluster biogenesis and iron trafficking 32,33

Buffer pH is typically kept between pH 6 and 8, with pH 7-8 promoting maximal capture of your his-tagged protein of interest, and pH 6-7 enhancing the purity of your eluted protein

Glutathione is one of the most popular and heavily-researched antioxidants around
